TGF-β1抗體說明書

【資料簡介】

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R&D Systems提供種類齊全的完整ELISA試劑盒。為確保高優品質及重復性，所有試劑盒均經過極其嚴格的質量檢驗。為檢測胞內和胞外蛋白，試劑盒使用了多種檢測手段，包括比色法、熒光法和化學發光法等。

規格：48次/96次        價格：1600/2600

 

 Goat Anti-TGF-β1

Catalog Number :HZ28554 Quantity size : 0.1ml （dilute with pH 7.4

0.01 M PBS or antibody diluent ） Background: Transforming growth factor βs （TGFβs） were originally discovered

due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. It is now realized that TGFβs mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGFβ1, TGFβ2 and TGFβ3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFβ requires dimerization of the monomers （usually homodimers） and release of the latent peptide portion. Overall, the mature region of the TGFβ3 protein has approximay 80% identity to the mature region of both TGFβ1 and TGFβ2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.

specificity :

              Anti-TGF-β1 is a Goat polyclonal antibody unconjugated.

              specific for TGF-β1 of human, mouse, rabbit

              use for western blotting,elisa,immunoprecipitation and immunohistochemistry

              Protein G affinity chromatography purification, purity :>95%

              Isotype: IgG

              Molecular Weight of TGFβ1 monomer: 12.5 kDa. Molecular Weight of TGFβ1 dimer: 25 kDa.

 

Application :

              Western blotting 1:100-500

              Immunohistochemistry 1:100-500

              ELISA 1:500-1000

              Optimal working dilutions must be determined by the end user. Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

 

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.