精品视频在线观看一区二区三区_v片免费看_欧美国产精品久久_国产真实乱免费高清视频_欧美精欧美乱码一二三四区_狠狠色婷婷j丁香综合社区

技術(shù)中心

CSB-E09033h人骨堿性磷酸酶(BALP)ELISA試劑盒說明書

2012年03月03日 14:33:45人氣:523來源:廈門慧嘉生物科技有限公司

資料類型doc文件資料大小120400
下載次數(shù)115資料圖片 【點(diǎn)擊查看】
上 傳 人廈門慧嘉生物科技有限公司 需要積分0
關(guān) 鍵 詞人骨堿性磷酸酶,BALP,ELISA試劑盒
【資料簡介】

 

 Human bone alkaline phosphatase (BALP) ELISA Kit
Catalog No. CSB-E09033h
(96 T)
This immunoassay kit allows for the in vitro quantitative determination of human BALP concentrations in serum, plasma a-n-d other biological fluids.
Expiration date six months from the date of manufacture
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
廈門慧嘉生物長期經(jīng)營ELISA試劑盒及Santa/Abcam抗體、Prospec細(xì)胞因子、Sigma/Amresco/Qiagen、Axygen耗材等生物試劑產(chǎn)品。誠信經(jīng)營,為客戶提供“zui高質(zhì)量的產(chǎn)品”和“zui的服務(wù)”:   :  1048735792 或登陸(向客服人員索取原版說明書)。歡迎廣告老師來詢!
 
PRINCIPLE OF THE ASSAY
The microtiter plate provided in this kit has been pre-coated with an antibody specific to BALP. Sta-n-dards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BALP a-n-d Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well a-n-d incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BALP, biotin-conjugated antibody a-n-d enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution a-n-d the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BALP in the samples is then determined by comparing the O.D. of the samples to the sta-n-dard curve.
DETECTION RANGE
3.12 ng/ml-200 ng/ml. The sta-n-dard curve concentrations used for the ELISA’s were 200 ng/ml, 100 ng/ml, 50 ng/ml, 25 ng/ml,
12.5 ng/ml, 6.25 ng/ml, 3.12 ng/ml.
SPECIFICITY
This assay recognizes recombinant a-n-d natural human BALP. No significant cross-reactivity or interference was observed.
SENSITIVITY
The minimum detectable dose of human BALP is typically less than 0 .78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
MATERIALS PROVIDED STORAGE

Reagent
Quantity
Assay plate
1
Standard
2
Sample Diluent
1 x 20 ml
Biotin-antibody Diluent
1 x 10 ml
HRP-avidin Diluent
1 x 10 ml
Biotin-antibody
1 x 120μl
HRP-avidin
1 x 120μl
 
1 x 20 ml
Wash Buffer
 
 
(25×concentrate)
TMB Substrate
1 x 10 ml
Stop Solution
1 x 10 ml

 
1.    Unopened test kits should be stored at 2-8?C upon receipt a-n-d the microtiter plate should be kept in a sealed bag. The test kit may be used throughout the expiration date of the kit, provided it is stored as prescribed above. Refer to the package label for the expiration date.
2.    Opened test plate should be stored at 2-8?C in the aluminum foil bag with desiccants to minimize exposure to damp air. The kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
3.    A microtiter plate reader with a ba-n-dwidth of 10 nm or less a-n-d an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.
 
REAGENT PREPARATION
Bring all reagents to room temperature before use.
1         Wash Buffer If crystals have formed in the concentrate, warm up to room temperature a-n-d mix gently until the crystals have compley dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer.
2         Sta-n-dard Centrifuge the sta-n-dard vial at 6000-10000rpm for 30s. Reconstitute the Sta-n-dard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 ng/ml. Allow the sta-n-dard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted sta-n-dard serves as the high sta-n-dard (200 ng/ml). The Sample Diluent serves as the zero sta-n-dard (0 ng/ml). Prepare fresh for each assay. Use within 4 hours a-n-d discard after use.
3         Biotin-antibody Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent(1:100), respectively.
4         HRP-avidin Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent(1:100), respectively.
 
Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, ha-n-d, face, a-n-d clothing protection when using this material.
OTHER SUPPLIES REQUIRED
1          Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
2          Pipettes a-n-d pipette tips.
3          Deionized or distilled water.
4          Squirt bottle, manifold dispenser, or automated microplate washer.
5          An incubator which can provide stable incubation conditions up to 37°C±0.5°C.
 
SAMPLE COLLECTION A-N-D STORAGE
Serum Use a serum separator tube (SST) a-n-d allow samples to clot for 30 minutes before centrifugation for 15 minutes at 1000 g. Remove serum a-n-d assay immediay or aliquot a-n-d store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
Plasma Collect plasma using citrate, EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 g within 30 minutes of collection. Assay immediay or aliquot a-n-d store samples at -20°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.
 
Note: Grossly hemolyzed samples are not suitable for use in this assay.
ASSAY PROCEDURE
Bring all reagents a-n-d samples to room temperature before use. It is recommended that all samples, sta-n-dards, a-n-d controls be assayed in duplicate. All the reagents should be added directly to the liquid level in the well. The pipette should avoid contacting the inner wall of the well.
1         Add 100μl of Sta-n-dard, Blank, or Sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37°C.
2         Remove the liquid of each well, don’t wash.
3         Add 100μl of Biotin-antibody working solution to each well. Incubate for 1 hour at 37°C. Biotin-antibody working solution may appear cloudy. Warm up to room temperature a-n-d mix gently until solution appears uniform.
4         Aspirate each well a-n-d wash, repeating the process three times for a total of three washes. Wash: Fill each well with
 
Wash Buffer (200μl) a-n-d let it sta-n-d for 2 minutes, then
remove the liquid by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel. Complete removal of liquid at each step is essential to good performance.
 
1         Add 100μl of HRP-avidin working solution to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
2         Repeat the aspiration a-n-d wash five times as step 4.
3         Add 90μl of TMB Substrate to each well. Incubate for 10-30 minutes at 37°C. Keeping the plate away from drafts a-n-d other temperature fluctuations in the dark.
4         Add 50μl of Stop Solution to each well when the first four wells containing the highest concentration of sta-n-dards develop obvious blue color. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
5         Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.
 
CALCULATION OF RESULTS
Using the professional soft "Curve Exert 1.3" to make a sta-n-dard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each sta-n-dard, control, a-n-d sample a-n-d subtract the average zero sta-n-dard optical density. Create a sta-n-dard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a sta-n-dard curve by plotting the mean absorbance for each sta-n-dard on the y-axis against the concentration on the x-axis a-n-d draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the BALP concentrations versus the log of the O.D. a-n-d the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the sta-n-dard curve must be multiplied by the dilution factor.
LIMITATIONS OF THE PROCEDURE
1          The kit should not be used beyond the expiration date on the kit label.
2          Do not mix or substitute reagents with those from other lots or sources.
3          It is important that the Sta-n-dard Diluent selected for the sta-n-dard curve be consistent with the samples being assayed.
4          If samples generate values higher than the highest sta-n-dard, dilute the samples with the appropriate Sta-n-dard Diluent a-n-d repeat the assay.
5          Any variation in Sta-n-dard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, a-n-d kit age can cause variation in binding.
6          This assay is designed to eliminate interference by soluble receptors, binding proteins, a-n-d other factors present in biological samples. Until all factors have been tested in the Quantikine Immunoassay, the possibility of interference cannot be excluded.
 
TECHNICAL HINTS
1          Centrifuge vials before opening to collect contents.
2          When mixing or reconstituting protein solutions, always avoid foaming.
3          To avoid cross-contamination, change pipette tips between additions of each sta-n-dard level, between sample additions, a-n-d between reagent additions. Also, use separate reservoirs for each reagent.
4          When using an automated plate washer, adding a 30 second soak period following the addition of wash buffer, a-n-d/or rotating the plate 180 degrees between wash steps may improve assay precision.
5          To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.
6          Substrate Solution should remain colorless or light blue until added to the plate. Keep Substrate Solution protected from light. Substrate Solution should change from colorless or light blue to gradations of blue.
7          Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution. Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

廈門慧嘉生物科技有限公司作者

上一篇:為什么聚丙烯酰胺PAM絮凝劑能夠處理低濁細(xì)泥?

下一篇:如何根據(jù)汽車涂裝廢水特點(diǎn)選擇合適的聚丙烯酰胺PAM型號?


我要投稿
  • 投稿請發(fā)送郵件至:(郵件標(biāo)題請備注“投稿”)hbzhan@vip.qq.com
  • 聯(lián)系電話0571-87759680
環(huán)保行業(yè)“互聯(lián)網(wǎng)+”服務(wù)平臺
環(huán)保在線APP

功能豐富 實(shí)時交流

環(huán)保在線小程序

訂閱獲取更多服務(wù)

微信公眾號

關(guān)注我們

抖音

環(huán)保在線網(wǎng)

抖音號:hbzhan

打開抖音 搜索頁掃一掃

視頻號

環(huán)保在線

公眾號:環(huán)保在線

打開微信掃碼關(guān)注視頻號

快手

環(huán)保在線

快手ID:2537047074

打開快手 掃一掃關(guān)注
意見反饋
主站蜘蛛池模板: 免费观看国产精品视频 | 欧美的一卡2卡3卡4卡5在线 | 网友自拍露脸国语对白 | 亚洲一区二区三区爽爽爽爽爽 | av观看成片免费网站 | 国产无吗一区二区三区在线欢 | 99久久精品久久 | 无码人妻丰满熟妇啪啪区日韩久久 | 狠狠操福利视频 | 欧美一级特黄aaaaaa在线看片 | 国产成人无码区免费内射一片色欲 | 色与欲影视天天看综合网 | 99国产精品视频免费观看一公开 | 亚洲十八爽 | 午夜理论欧美理论片 | 亚洲av午夜成人片动漫番 | 国产一区二区三区四区五区tv | x8x8女性性爽爽在线观看 | 国产欧美VA欧美VA在线 | 在线观看免费视频亚洲 | 久久国产在线视频 | 天天搞夜夜 | 欧美精品一二三 | a色综合 | 日本少妇翘臀啪啪无遮挡 | av片免费大全在线观看不卡 | 亚洲日韩精品一区二区三区无码 | 伊人开心22.yiren亚洲 | 亚洲精品午夜一区人人爽 | 亚洲黄色在线视频 | 久久免费看少妇高潮a片特黄 | 久久精品a一级国产免视看成人 | 亚洲熟女综合色一区二区三区 | 国产精品高潮呻呤 | 办公室被绑奶头调教羞辱OL | 精品一区二区视频在线 | 人妻有码中文字幕在线 | 亚洲一区二区三区在线网站 | 亚洲精品中文字幕久久久久 | 久久成年片色大黄全免费网站 | 日韩亚洲在线视频 |