牛維生素B12(VB12)ELISA Kit

產品類型:
ELISA Kit

產品名稱:

牛維生素B12(VB12)ELISA Kit

英文名稱:

Bovine Vitamin B12,VB12 ELISA Kit

牛維生素B12(VB12)ELISA Kit規格:
96T
牛維生素B12(VB12)ELISA Kit種屬:
Bovine
待測物名稱:
Vitamin B12,VB12
縮寫:
VB12
蛋白功能1:
Metabolism
檢測范圍:
0.312pg/ml-20pg/ml
靈敏度:
0.299 pg/ml
反應時間:
1-5h
所需樣本體積:
50-100ul
檢測波長:
450 nm

牛維生素B12(VB12)ELISA Kit    This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.